rabbit anti abca1 pab (Novus Biologicals)
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Rabbit Anti Abca1 Pab, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+abca1/ABCA1+Antibody+-+BSA+Free/pmc12795503-160-48-54
Average 94 stars, based on 397 article reviews
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other:Article Title: Effect of Myelin Debris on the Phenotypic Transformation of Astrocytes after Spinal Cord Injury in Rats. Article Snippet: After spinal cord injury (SCI), the accumulation of myelin debris can serve as proinflammatory agents, hindering axon regrowth and exacerbating damage.. While astrocytes have been implicated in the phagocytosis of myelin debris, the impact of this process on the phenotypic transformation of astrocytes and their characteristics following SCI in rats is not well understood.. Here, we demonstrated that the conditioned medium of myelin debris can trigger apoptosis in rat primary astrocytes in vitro. Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration. Article Snippet: The following antibodies were used: rat antimyelin basic protein (MBP, 1:100, Bio-Rad antibodiesMCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse antiNestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, SigmaAldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, EnzoADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), Article Title: Astrocyte-Oligodendrocyte interaction regulates central nervous system regeneration Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) and anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), Article Title: Berberine attenuates diabetic atherosclerosis via enhancing the interplay between KLF16 and PPARα in ApoE -/- mice. Article Snippet: Cardiovascular disease caused by atherosclerosis is a leading cause of morbidity and mortality worldwide.. Diabetes is a major independent risk factor for the development of atherosclerotic cardiovascular diseases.. Diabetic atherosclerosis is characterized by hyperglycemia, hyperinsulinemia, and dyslipidemia. Article Title: Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration Article Snippet: The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG, 1:100, Sigma-Aldrich MAB1567), chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500, Cambridge Bioscience 829401), rabbit anti-SOX9 (1:500, Sigma-Aldrich AB5535), mouse anti-Nestin (1:100, Abcam ab6142), mouse anti-vimentin (1:100, Sigma-Aldrich clone LN-6 MAB1681), rabbit anti-nuclear factor IA (NFIA, 1:250, Abcam ab228897), rabbit anti-Ki67 (1:500, Sigma-Aldrich AB9260), mouse anti-APC (CC1, 1:100, Abcam ab16794), rabbit anti-Olig2 (1:100, Sigma-Aldrich AB9610), rabbit anti-HMOX1 (1:100, Enzo ADI-SPA-895-D), rabbit anti-HMGCS1 (1:500, Invitrogen PA5-29488), rabbit anti-FDPS (1:500, Invitrogen PA5-28228), anti-MVD antibody (1:100, Abcam ab198823) rabbit anti-FDFT1 (1:100, Abcam ab236666), rabbit anti-IBA1 (1:500, Abcam 178846), Article Title: Eicosapentaenoic acid inhibits cholesterol efflux pathways from cholesterol-loaded human THP-1 macrophages by reducing the hydrolysis of cholesteryl esters mediated by carboxylesterase 1. Article Snippet: A diet high in n-3 polyunsaturated fatty acids (PUFAs), particularly eicosapentaenoic acid (EPA) (C20:5 n-3), is cardioprotective.. PUFAs integrate into membrane phospholipids, altering membrane protein function.. We investigated the effects of various PUFAs on the anti-atherogenic cholesterol efflux pathways from cholesterolloaded human THP-1 macrophages. Bioprocessing:Article Title: Regulation of astrocyte lipid metabolism and ApoE secretionby the microglial oxysterol, 25-hydroxycholesterol Article Snippet: .. Mouse anti-human ApoE monoclonal antibodies (HJ15.7 and biotinylated-HJ15.4; ( 27 )) and mouse anti-mouse ApoE monoclonal antibodies (HJ6.2, HJ6.3, and biotinylated-HJ6.8; ( 28 )) were from the Holtzman lab. Article Title: Regulation of astrocyte lipid metabolism and ApoE secretionby the microglial oxysterol, 25-hydroxycholesterol Article Snippet: .. Antibodies Mouse anti-human ApoE monoclonal antibodies (HJ15.7 and biotinylated-HJ15.4; ( 27 )) and mouse anti-mouse ApoE monoclonal antibodies (HJ6.2, HJ6.3, and biotinylated-HJ6.8; ( 28 )) were from the Holtzman lab. |
![( A ) Enhancement of growth inhibitory effect of NC on human renal carcinoma cells by co-treatment with an <t>ABCA1</t> inhibitor cyclosporin A (CsA). Cells were grown with or without 20 μM NC and 10 μM CsA for 24 h, and cell number was determined with CCK-8. Fold change in cell number by NC was shown as relative to controls without NC. Results are shown as mean of at least four independent experiments ± SD (Tukey–Kramer’s multiple comparison test). n = 4 (KMRC-3,786-O,A498), 5 (Caki-1) or 6 (TUHR14TKB). ( B–E ) Immunoblot analysis of whole-cell lysate of human renal carcinoma cell lines. A representative result from three independent experiments is shown ( B ). Quantitative analyses of Snail ( C ) and ABCA1 ( D ) expression are presented. A scatter plot illustrating the correlation between ABCA1 and Snail expression levels is also shown ( E ). Quantitative data are shown as mean of three independent experiments ± SD (Tukey–Kramer’s test). ( F ) Comparison of ABCA1 expression between normal tissue and primary tumor of indicated subtypes of renal cancers analyzed using UCSC Xena ( https://xenabrowser.net/ ). Bars indicate means (Welch’s t- test). ( G ) Immunohistochemistry of a surgically extracted renal tissue from a patient with Fuhrman grade 3 primary ccRCC, indicating upregulation of ABCA1 in the lesion site. The ABCA1 staining was verified by the accumulation of known signals in renal tubules and glomeruli in normal tissue . Scale bars, 250 μm (left), 50 μm (right). ( H ) Quantification of ABCA1 signal from surgically extracted renal tissues from three patients for each Fuhrman grade (Tukey–Kramer’s test; see also ). ( I ) Phase-contrast images of EpH4 wild-type cells (top) and Snail-overexpression cells (bottom, EpH4-Snail). Scale bars, 50 μm. ( J ) Immunofluorescence images of EpH4 and EpH4-Snail cells. The cells were fixed. Scale bars, 20 μm. ( K ) Acquisition of NC resistance by exogenous expression of Snail in EpH4 cells and enhancement of 20 μM NC effect on EpH4-Snail cells by co-treatment with 10 μM CsA. Data are presented as in ( A ) ( n = 3, Tukey–Kramer’s test). ( L ) Immunoblot analyses of whole-cell lysates of EpH4, EpH4-Snail, E-cadherin KO, and α-catenin KO EpH4 cells. Quantitative analyses of ABCA1 expression is also shown ( n = 3, Dunnett”s test). ( M ) Immunofluorescence images of EpH4 and EpH4-Snail cells. Cells were fixed with MeOH. Scale bars, 20 μm. ( N, O ) Immunoblot analyses of whole-cell lysates of MDCK II cells ( N ) expressing KRAS G12V treated with 5 μg/ml TGFβ for 0, 3, and 8 days and human esophageal carcinoma cell lines TE-15 and TE-8 ( O ). Graphs on the bottom show analyses from three biological replicates (Tukey–Kramer’s test [ N ] and Student’s t -test [ O ]). Phase-contrast images are also shown on the top ( O ). Scale bars, 50 μm. Figure 1—source data 1. Original raw data for the immunoblot images shown in . Figure 1—source data 2. Labeled full blot images for the immunoblot images shown in .](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5503/pmc12795503/pmc12795503__elife-104374-fig1.jpg)
